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Sedimentation velocity and dynamic light scattering analyses of Sp-DHDPS.

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posted on 13.12.2013, 02:44 by Con Dogovski, Michael A. Gorman, Natalia E. Ketaren, Judy Praszkier, Leanne M. Zammit, Haydyn D. Mertens, Gary Bryant, Ji Yang, Michael D. W. Griffin, F. Grant Pearce, Juliet A. Gerrard, Geoffrey B. Jameson, Michael W. Parker, Roy M. Robins-Browne, Matthew A. Perugini

(A) Continuous sedimentation coefficient, c(s), distribution of Sp-DHDPS at 4.5 µM plotted as a function of standardized sedimentation coefficient (s20,w) with RMSD and runs test Z values for the best-fit of 0.008 and 6.6, respectively. (B) Continuous mass, c(M), distribution of Sp-DHDPS at a concentration of 4.5 µM plotted as a function of molar mass with RMSD and runs test Z values of 0.008 and 6.6 respectively. (C) c(s) distribution of Sp-DHDPS at 740 nM, with RMSD and runs test Z values of 0.02 and 1.64, respectively. Data were analyzed employing the program SEDFIT [51,52,65,66] using a resolution (N) of 200 and a sedimentation coefficient range of 0.1-12 S or molar mass range 1.0-250 kDa with a P-value of 0.95. Above Panels A-C: Residuals plotted as a function of radial position resulting from continuous size-distribution best-fits shown in panels A-C. (D) A distribution plot of the average unweighted hydrodynamic radii of Sp-DHDPS at a concentration of 59 µM determined using dynamic light scattering (DLS). The results of the distribution plot reveal an average hydrodynamic radius of 4.5 ± 0.2 nm.

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