Sedimentation velocity analysis of ScRIB7.
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A, The raw sedimentation signals acquired at different time points. Protein sample was prepared as OD280 absorbance about 0.8 in buffer containing 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl. The sample was scanned at intervals of 30 s for 4 h. (b) Continuous molar mass distribution of the protein showing a single peak with a molecular mass of ∼43 kDa, which is closest to the mass of a ScRIB7 dimer.