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Sedimentation velocity analysis of ScRIB7.

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posted on 19.04.2013 by Zongyang Lv, Jian Sun, Yingfang Liu

A, The raw sedimentation signals acquired at different time points. Protein sample was prepared as OD280 absorbance about 0.8 in buffer containing 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl. The sample was scanned at intervals of 30 s for 4 h. (b) Continuous molar mass distribution of the protein showing a single peak with a molecular mass of ∼43 kDa, which is closest to the mass of a ScRIB7 dimer.