Secondary screening of hit compounds against multiple RNA viruses and validation determined by dose response curve analysis of lead compounds and reference compounds.
(A) Heat map showing relative percent infection inhibition (left panel) and percent cell number (right panel) of compounds (10 µM concentrations) against multiple RNA viruses. The four hit compounds are marked with arrows (A) and their primary structures are shown (B). (C) Dose response curve analysis of the four lead compounds and the reference compounds ribavirin and IFN-α2a. Percent infection inhibition is shown in blue and the percent cell number is shown in red.