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Screening of viral proteins or viral replicative intermediate dsRNA involved in induction of COX-2/ IL-32.

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posted on 21.02.2013, 10:37 by Wei Li, Yan Liu, Muhammad Mahmood Mukhtar, Rui Gong, Ying Pan, Sahibzada T. Rasool, Yecheng Gao, Lei Kang, Qian Hao, Guiqing Peng, Yanni Chen, Xin Chen, Jianguo Wu, Ying Zhu

Reporter plasmids pCOX-2-Luc (A), pIL-32-Luc (B), and pRL-TK were cotransfected along with all 10 viral gene constructs (Flag2A-HA, Flag2A-NA, Flag2A-NP, Flag2A-NS1, Flag2A-NS2, Flag2A-M1, Flag2A-M2, Flag2A-PA, Flag2A-PB1, Flag2A-PB2) and control vectors (Flag2A) or treated with or without poly(IC) (50 µg/ml), poly(IC) (50 µg/ml)+IFN-γ (150 U/ml) into A549 cells which are indicated on the horizontal axis respectively. Luciferase activity was measured as described in methods section. Results are expressed as the mean±s.d. of three independent experiments performed in triplicate and normalized by Renilla activities.