Screening for biologically active fractions isolated from 15LO-oxidized AA-CE.
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The product of 15LO-mediated oxidation of AA-CE was separated using one-step normal phase LC as described in Methods. The collected fractions were added to J774 macrophages for 15 min, and cell spreading was scored by two independent observers, with selected samples photographed in phase contrast. Cell lysates were analyzed for p-ERK1/2 and total ERK1/2 in immunoblot. Cells treated with media only and with mmLDL were used as negative and positive controls.