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Schematic representation of the expression strategy.

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posted on 27.09.2013, 01:54 by Paola Rognoni, Francesca Lavatelli, Simona Casarini, Giovanni Palladini, Laura Verga, Paolo Pedrazzoli, Giovanna Valentini, Giampaolo Merlini, Vittorio Perfetti

Each patient’s full-length monoclonal LC (VL +CL) obtained by RT-PCR was re-amplified using primers designed according to the pASK-IBA cloning strategy. Start Met codon in 5′ primer and the two stop codons in 3′ primer were introduced and shown in bold, BsmBI recognition sites are indicated in lower case in both primers. After BsmBI digestion, PCR product was ligated into BsaI/BsaI sites of pASK-IBA33plus vector.

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