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Schematic illustration of His-tagged membrane proteins on Ni2+-coated plate.

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posted on 20.02.2013, 15:01 by Hyun-Ho Lim, Yiling Fang, Carole Williams

Since AdiC and CLC-ec2 form stable dimers, a single His-tag system could be better to expose both cis- and trans-sides to the solution (right) than doubly tagged dimer (left).

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