Schematic and graphs illustrating the experimental approach (A) and results (B–E) for animals treated with vehicle followed by CORT.
A: Experimental protocol for the vehicle-first treatment regimen. Video-EEG was monitored continuously throughout the entire regimen, with each period corresponding to 1–2 weeks. Twenty-four hour EEG segments quantified for epileptiform activity are denoted by arrows. The color of each part of the timeline corresponds to the bar color in the graphs shown below. B: CORT treatment significantly increased the number of epileptiform events relative to all other periods. C: CORT treatment significantly increased the total duration of epileptiform events relative to all other periods. Vehicle treatment also significantly increased the duration of events relative to the 1st baseline period. D: No significant effects of vehicle or CORT treatment on seizure frequency were found. E: Mean seizure duration was statistically equivalent among treatment groups and baseline periods. Bar graphs show means ± SEM. *, p<0.05.