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Saturation binding assays of wild type TP and the mutant receptors using the TP antagonist [3H] SQ 29,548.

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posted on 2012-01-17, 00:38 authored by Raja Chakraborty, Sai Prasad Pydi, Scott Gleim, Shyamala Dakshinamurti, John Hwa, Prashen Chelikani

Saturation assays with membrane bound TP and the mutant receptors, TM4 mutants in panel A and TM3 mutants in panel B, were performed with different concentrations of [3H] SQ 29,548. A one way ANOVA analysis without any post hoc test showed that except for G164V in TM4 there were no significant difference between TP and the mutants at significance level of p<0.05. The data is from a minimum of three independent experiments, with each point in duplicate.