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Salmonella-specific Th1 cells target flagellin and T3SS effectors at different time points after infection.

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posted on 20.02.2013, 09:48 by Seung-Joo Lee, James B. McLachlan, Jonathan R. Kurtz, Danhua Fan, Sebastian E. Winter, Andreas J. Baumler, Marc K. Jenkins, Stephen J. McSorley

C57BL/6 mice were infected intravenously with 5×105 Salmonella (BRD509). On day 7 or day 40 post-infection, CD4 T cells from the spleens were purified and restimulated with 10 µM of various peptides (Table 3) for 16 h in the presence of irradiated splenocytes. IFN-γ production was measured by ELISPOT assay. (Top) Representative images from IFN-γ ELISPOT plates using CD4 T cells from Salmonella-infected mice at day 7 or day 40 after infection. (Bottom) Scatter graphs show the number of IFN-γ-producing CD4 T cells per infected mouse. Numbers above indicate statistical significance and show p value of a comparison between media alone (no stimulation) and peptide stimulation group.