S. pneumoniae serotype 14 day 1, 3, 5 and 7 biofilms imaged using (a) scanning electron microscopy with Alcian Blue staining (8,000x magnification; scale bar: 5 µm), and (b) confocal microscopy using Live/Dead staining, with sideviews (YZ – right) and (XZ – bottom) sagittal sections of the biofilm.
Scale bar beside YZ sagittal sections represents average biofilm thickness. Scale bar in xy pane: 30 µm. (c) Bar chart comparing the total number of cells between day 1, 3, 5 and 7 biofilms. (d) Bar chart comparing number of viable cells between day 1, 3, 5 and 7 biofilms through CFU cm−2 measurement.