SVV infection of rhesus macaques elicits a robust peripheral T cell response.
CD4 and CD8 T cells were separated into three subsets based on expression of CD28 and CD95: naïve (CD28+CD95−); central memory (CM, CD28+CD95+); and effector memory (EM, CD28−CD95+) cells. A representative profile of CD8 T cell subsets of monkey 24953 on 0 dpi is shown (A, left). T cell proliferation was measured as described in Figure 3 based on expression of the nuclear protein Ki67 using flow cytometry. T cell proliferation dramatically increased 14 dpi (A, right) compared to 0 dpi (A, middle). Fold increases (relative to 0 dpi) in the numbers of Ki67+ CD4 CM and EM (B and C) and CD8 CM and EM T cells (D and E) in PBMCs indicate a peak proliferative response of all T cell subsets 14 dpi in all monkeys (B–E).