STS treatment suppresses expression of phosphorylated Smad3 in the PBOO model.
(A) Representative phosphorylated Smad3-immunostained rat bladder sections. (a) Sham group at 4 weeks, (b) week 4 after PBOO, (c) week 4 after PBOO+STS treatment, (d) week 8 after Sham group, (e) week 8 after PBOO, (f) week 8 after PBOO+STS treatment. Expression of phosphorylated Smad3 was significantly higher in PBOO rats at weeks 4 and 8 compared with the Sham group (P<0.05, n = 8). STS treatment induced decreased expression of phosphorylated Smad3 (P<0.05, n = 8). (B) Densitometric analyses performed from 8 independent experiments. Each bar represents the mean±SD of 8 rats. *P<0.05 vs. sham group (n = 8); #P<0.05 vs. PBOO group. Original magnifications, ×400. IOD, integrated optical density.