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SP-D expression in EDE before and after P.aeruginosa challenge.

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posted on 06.06.2013, 00:49 by Susan R. Heimer, David J. Evans, James J. Mun, Michael E. Stern, Suzanne M. J. Fleiszig

Western immunoblot blot analysis of SP-D expression in pooled ocular surface washes from EDE and control mice (10 mice per group) after 5 days EDE induction, and before and 6 h after inoculation with P. aeruginosa strain PAO1 (109 cfu). To normalize for differences in tear volume, equivalent amounts of protein (2 µg) were used in the analysis (BCA protein assay). Purified recombinant SP-D (rSP-D, ∼43 kDa monomer), and a relevant number of bacteria suspended in PBS (5×103 cfu, see Fig. 2B), were included as positive and negative controls, respectively. SP-D expression in ocular surface washes was increased under EDE conditions before bacterial inoculation. The experiment was repeated once.