SPR analysis of the interaction of adenine nucleotides to immobilized. M.leprae HSP18 interactions.
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posted on 2015-03-26, 04:46 authored by Sandip Kumar Nandi, Ayon Chakraborty, Alok Kumar Panda, Sougata Sinha Ray, Rajiv Kumar Kar, Anirban Bhunia, Ashis BiswasSensorgrams (solid line) of the binding of (A) ATP (0.1–5 μM); (B) ATP-γS (0.1–1 μM); (C) ADP (0.1–100 μM) and (D) AMP (0.1–100 μM) to M. leprae HSP18 covalently captured on a sensor chip at 25°C.
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antigenic proteins actchaperone functionheat shock proteinComparative sequence alignmentSmall Heat Shock ProteinHSP 18Molecular docking studiesMycobacterium leprae bacillisequence 49 KADSLDIDIE 58leprae HSP 1818 kDa antigenATP triggers exposureleprae HSP 18. ATP perturbssurface plasmon resonance measurement
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