Figure_5.tif (1.02 MB)
Download file

SKAP interacts with Prp19 in vivo.

Download (0 kB)
figure
posted on 09.04.2014, 03:37 by Shan Lu, Renxian Wang, Congli Cai, Junbo Liang, Longchang Xu, Shiying Miao, Linfang Wang, Wei Song

(A and B) Co-immunoprecipitation assays for SKAP and Prp19. HEK-293T cells were transfected with Flag-SKAP and Myc-Prp19. After immunoprecipitation with anti-Myc beads, Flag-SKAP was detected by immunoblotting using an anti-Flag antibody; after immunoprecipitation with anti-Flag beads, Myc-Prp19 was detected by immunoblotting using an anti-Myc antibody. (C) Immunoprecipitation assays for SKAP and Prp19. HEK-293T/Flag-strepII-RFP and HEK-293T/Flag-strepII-SKAP stable cell lysates were incubated with anti-Flag beads; endogenous Prp19 was detected by immunoblot using an anti-Prp19 antibody.

History