SKAP interacts with Prp19 in vivo.
(A and B) Co-immunoprecipitation assays for SKAP and Prp19. HEK-293T cells were transfected with Flag-SKAP and Myc-Prp19. After immunoprecipitation with anti-Myc beads, Flag-SKAP was detected by immunoblotting using an anti-Flag antibody; after immunoprecipitation with anti-Flag beads, Myc-Prp19 was detected by immunoblotting using an anti-Myc antibody. (C) Immunoprecipitation assays for SKAP and Prp19. HEK-293T/Flag-strepII-RFP and HEK-293T/Flag-strepII-SKAP stable cell lysates were incubated with anti-Flag beads; endogenous Prp19 was detected by immunoblot using an anti-Prp19 antibody.