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SIRT1 affects ACC and AMPK phosphorylation levels after t10c12 CLA treatment.

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posted on 2012-11-14, 00:49 authored by Shan Jiang, Wei Wang, Jess Miner, Michael Fromm

Differentiated 3T3-L1 adipocytes were incubated with 100 µmol/L LA (L) or t10c12 CLA (C): a with or without SIRT1 activator SRT1720 (SRT), or sirtuin inhibitors sirtinol (SOL) or nicotinamide (NAM) for 2 or b 12 h; or c with 40 nmol/L of SIRT1 siRNA (siSIRT1) or control siRNA (siCON). Representative western blots indicate the proteins detected in cytosolic extracts with antibodies to p-ACC (p-Ser79), or total ACC, p-AMPK (p-Thr172), or total AMPK. Each bar in panels a–c represents the mean + SEM (n = 3) of the ratio of the phosphorylated to total form of each protein (phospho/total) for three independent experiments. Means for p-AMPK/AMPK (a–e) or p-ACC/ACC (u–x) not sharing a common letter differ, P0.05.

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