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SELEX evolution by ELONA assay.

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posted on 2012-04-17, 00:57 authored by Pedro Nadal, Alessandro Pinto, Marketa Svobodova, Nuria Canela, Ciara K. O'Sullivan

Evaluation of evolution using ELONA monitoring of interaction between immobilised target and DNA isolated after rounds 4 (R4), 7 (R7), 10 (R10), 12 (R12) and 14 (R14), as compared to the interaction with the initial library. A control in the absence of coated target for each of the rounds (No coating) and a background control that refers to the response in the presence of buffer alone (Buffer).

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