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SEC chromatograms of NLP assemblies at various lipid-to-apoLp-III molar ratios.

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posted on 2010-07-19, 00:08 authored by Nicholas O. Fischer, Craig D. Blanchette, Brent W. Segelke, Michele Corzett, Brett A. Chromy, Edward A. Kuhn, Graham Bench, Paul D. Hoeprich

NLPs were assembled with DMPC and apoLp-III from A) Bombyx mori or B) Manduca sexta. SEC traces are parsed into 6 distinct regions: 1, lipid-rich; 2–5, NLPs; 6, protein-rich. Exclusion limit of the column (Vo) and molecular weights of protein standards are indicated.