SDS-patient SBDS proteins are localized to the nucleus.
(A) Schematic overview of the GFP-tagged SBDS constructs (B) Western blot analysis shows that GFP-tagged SBDS proteins have the expected molecular sizes of 59 kDa, 36 kDa, 39 kDa and 54 kDa for the GFP-SBDS-FL, GFP-SBDS-K62, GFP-SBDS-C84 and GFP-SBDS-R218 respectively. (C) Representative pictures of the intracellular localization of the GFP-tagged SBDS proteins. Bottom panel shows the GFP fluorescence intensity plots measured as indicated in the corresponding cells in the top panel. (D) Average ratio of the nuclear/cytoplasmic GFP fluorescence intensity for the different GFP-tagged constructs Asterisk indicates that the localization of the GFP-SBDS-FL is statistically significant different (p<0.001) from the SDS-patient GFP-SBDS proteins. Error bar indicates s.e.m.(FL n = 42 cells, K62 n = 52 cells, C84 n = 37 cells, R218 n = 33 cells analysed in 3–5 independent experiments).