Fig_8.tif (448.84 kB)
Download file

SDS-PAGE profile of trypsin digestion of M. leprae HSP18 in the absence and presence of 1 mM ATP.

Download (0 kB)
posted on 26.03.2015, 04:46 by Sandip Kumar Nandi, Ayon Chakraborty, Alok Kumar Panda, Sougata Sinha Ray, Rajiv Kumar Kar, Anirban Bhunia, Ashis Biswas

(A) HSP18 (0.5 mg/ml) in 50 mM phosphate buffer, pH 7.5 was digested with trypsin at 100:1 ratio (w/w) for different times at 37°C. All odd-numbered lanes had no ATP, and even-numbered lanes had 1 mM ATP. (B) Bar diagram representation of the net cleavage of M. leprae HSP18 by trypsin at different time points. ***p<0.0005