SDS-PAGE profile of trypsin digestion of M. leprae HSP18 in the absence and presence of 1 mM ATP.
(A) HSP18 (0.5 mg/ml) in 50 mM phosphate buffer, pH 7.5 was digested with trypsin at 100:1 ratio (w/w) for different times at 37°C. All odd-numbered lanes had no ATP, and even-numbered lanes had 1 mM ATP. (B) Bar diagram representation of the net cleavage of M. leprae HSP18 by trypsin at different time points. ***p<0.0005