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SDS-PAGE of T. cruzi subcellular fractions.

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posted on 18.09.2015, 03:16 by Agenor de Castro Moreira dos Santos Júnior, Dário Eluan Kalume, Ricardo Camargo, Diana Paola Gómez-Mendoza, José Raimundo Correa, Sébastien Charneau, Marcelo Valle de Sousa, Beatriz Dolabela de Lima, Carlos André Ornelas Ricart

Epimastigote cells were lysed and subjected to cell fractionation as described in Material and Methods. Cytosolic, kinetoplastic and nuclear samples (30μg/lane) were subjected to 13% T SDS-PAGE. The gel was stained with Coomassie Brilliant Blue.

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