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SDS-PAGE of IgG cleavage with endopeptidase IdeE and IdeE2.

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posted on 21.02.2013 by Bengt Guss, Margareta Flock, Lars Frykberg, Andrew S. Waller, Carl Robinson, Ken C. Smith, Jan-Ingmar Flock

(A) IdeE cleavage of human IgG in the presence of serum from Septavacc vaccinated ponies and (B) IdeE2 cleavage of horse IgG in the presence of serum from Septavacc vaccinated ponies. (A) Lane 1; Septavacc diluted 4 times, lane 2; Septavacc serum diluted 8 times, lane 3; Septavacc serum diluted 16 times, lane 4; Septavacc serum diluted 32 times, lane 5; Septavacc serum diluted 64 times, lane 6; human IgG+IdeE, lane 7; human IgG, lane 8; size marker (in kDa 97, 66, 45, 30, 20, 14,4). The same amount of IdeE is added to sample 1–6 and the same amount of human IgG is added to sample 1–7. (B) Lane 1; Septavacc undiluted, lane 2; Septavacc diluted 2 times, lane 3; Septavacc diluted 4 times, lane 4; Septavacc diluted 8 times, lane 5; Septavacc diluted 16 times, lane 6; horse IgG+IdeE2, lane 7; horse IgG, lane 8; size marker. The same amount of IdeE2 was added to sample 1–6 and the same amount of horse IgG was added to sample 1–7. The arrows just above the 30 kDa band indicate the cleavage product of IgG.

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