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S1, S2, S3 and S4 DNA are inert and inhibit HMGB1, but not LPS-induced TNF release from macrophages.

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posted on 15.08.2014, 03:35 by Zhongliang Ju, Sangeeta S. Chavan, Daniel J. Antoine, Meghan Dancho, Teá Tsaava, Jianhua Li, Ben Lu, Yaakov A. Levine, Andrew Stiegler, Yehuda Tamari, Yousef Al-Abed, Jesse Roth, Kevin J. Tracey, Huan Yang

A) S1–S4 DNA is inert. Primary human macrophages were incubated with HMGB1 (positive control) or S1–S4 DNA (1 µM) for 16 hours. TNF released in the supernatants was measured by ELISA. Data are mean ± SEM from 3 experiments. B) S1–S4 DNA inhibits HMGB1-induced TNF release from cultured macrophages. Primary human macrophages in 96-well culture plates were stimulated with HMGB1 and various amounts of DNA as indicated for 16 hours. TNF released in the supernatants were measured by ELISA. N = 3 independent experiments. *: p<0.05 vs. HMGB1 alone. C) S1–S4 DNA does not inhibit LPS-induced TNF release. Primary human macrophages in 96-well culture plates were stimulated with LPS (2 ng/ml) plus increasing amounts of S1–S4 DNA overnight at 37°C. TNF released was measured. N = 3 experiments.

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