Root Mean Square Fluctuations in clusters and specific systems.
a) Cα atom RMSF in the 3 clusters obtained by clustering. Cyan dashed line: cluster #1 (i.e. frames from the F429A, F429E and F429H simulations); full black line: cluster #2 (WT); magenta dot-dashed line: cluster #3 (F429L). b) difference of RMSF calculated from the trajectories of 4 mutants with respect to the WT trajectory. Full blue line: F429A; magenta dashed line: F429E; orange dot-dashed line: F429H; green dotted line: F429L.