Rim101 in low iron media.
A. Rim101 is required for growth in low-iron medium. Strains were incubated in low-iron medium and growth was quantified by monitoring the absorbance of the culture at 600 nm. B. The 5′-untranslated regions of C. neoformans genes involved in iron regulation were evaluated for the presence of putative Rim101 binding sites (GCCAAG or CCAAGAA).