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RickA expression of rickettsiae grown in different cell lines.

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posted on 09.07.2008, 02:08 by Premanand Balraj, Khalid El Karkouri, Guy Vestris, Leon Espinosa, Didier Raoult, Patricia Renesto

(A) Vero cells (V) or L929 cells (L) infected with R. raoultii (Rra) or R. conorii (Rco) were subjected to SDS-PAGE and then transferred to a nitrocellulose membrane. The membranes were subsequently probed with anti-RickA or anti-Adr1 antibodies. Identical amounts of proteins were loaded in each lane. Bartonella quintana (Bq) whole extracts were used as a negative control. (B) Quantification of RickA expression normalized to Adr1 expression for each sample. Data are mean+/−SEM for three distinct experiments.


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