Reservatrol-mediated upregulation of DR5.

(A) SUDHL4 cells were treated with 50 µM Resveratrol for indicated time periods. After cell lysis, equal amounts of proteins were immuno-blotted with antibodies against DR5 and beta actin. (B) SUDHL4 and HBL-1 cells were pre-treated with either 80 µM of z-VAD (upper panel) or 10mM NAC (lower panel) for 2 hours and subsequently treated with 50 µM Resveratrol for 24 hours. Cells were lysed and equal amounts of proteins were immunoblotted with antibodies against DR5 and beta-actin. (C) SUDHL4 cells were either transfected with 50 and 100nM siRNA, specific against DR5 or scrambled siRNA for 48 hours. Cells were then treated with 25 and 50 µM Resveratrol for 24 hours, following which cells were stained with fluorescent-conjugated Annexin V/PI and analyzed by flow cytometry. (D) SUDHL4 cells were either transfected with 100nM siRNA, specific against DR5 or scrambled siRNA for 48 hours and treated with 25 and 50 µM Resveratrol for 24 hours. Cells were lysed and equal amounts of proteins were immuno-blotted with antibodies against DR5, caspase-3, cleaved caspase-3, PARP and beta-actin.