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Requirements for ionic and hydrophobic interactions for processing of Pro-FP3.

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posted on 2013-02-19, 21:38 authored by Srinivasan Sundararaj, Deepak Singh, Ajay K. Saxena, Kapil Vashisht, Puran S. Sijwali, Rajnikant Dixit, Kailash C. Pandey

A. The wild FP3 and mutated enzyme, Glu 238 and Phe 231 were expressed in E.Coli, purified and refolded, and finally processed and compare with wild FP3 . The enzymes were further analyzed by SDS-PAGE and Western blot. B. The wild FP3 and mutated enzymes (Phe 231, Glu 238) were processed and subjected to gelatin substrate native PAGE, and assess their functional activity.