Requirement of virus replication for accumulation of lipidated LC3.
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A–B) Western blots of extracts from MA104 cells infected or not with antigenically intact inactivated RV particles (i-OSU or i-SA11, 13 hpi) and untreated or treated with MG132 (5 µM, added at 1 h before infection) in A and with CQ (50 µM, added at 1 h after infection) in B. Since virus inactivation was performed on crude preparations, equal amounts of lysates derived from non-infected cells were psoralen-treated and UV-exposed and used as mock-infection controls (i-mock). C) Western blot of extracts from MA104 cells transfected with the indicated siRNAs and infected with RV (OSU strain; MOI, 5; 13 hpi) at 48 h after transfection.