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Representatives of C-di-GMP specific domain architectures in Mycobacteirum tuberculosis and their subsequent protein purification.

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posted on 2010-11-30, 01:47 authored by Kajal Gupta, Prasun Kumar, Dipankar Chatterji

(A) Domain architecture of the protein with GGDEF and EAL domain in Mycobacterium tuberculosis. The length of each sequence is approximately to scale. (B) Purification of C- terminal His6 tag proteins Lane – 1 Marker, Lane - 2 MtbDGC and Lane – 3 MtbPDE. C-terminal His6 –tagged Rv1354 is 67.6 KDa and Rv1357c is 33.9 KDa. Purity of the preparations was determined by (10%) SDS- PAGE.