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Representative images of U-2 OS cells treated with 100 nM epothilone B or pretubulysin for 48 h.

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posted on 17.05.2012, 02:13 by Jennifer Herrmann, Yasser A. Elnakady, Romina M. Wiedmann, Angelika Ullrich, Manfred Rohde, Uli Kazmaier, Angelika M. Vollmar, Rolf Müller

Nuclei were stained with Hoechst 33342 (pseudocolor blue, left panel), and γH2A.X was visualized by immunofluorescence (pseudocolor red, right panel). Arrows indicate fragmented nuclei with bright fluorescent spots due to H2A.X phosphorylation events. Images were acquired on a BD Pathway 855 automated microscope and subsequently processed and analyzed in AttoVision v1.6.2 (the full HCS analysis is provided as supplementary material, Figure S2).