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Replication defective ZEBRA mutants activate brlf1 gene expression to wild-type levels.

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posted on 2010-08-19, 01:26 authored by Ayman El-Guindy, Lee Heston, George Miller

Total RNA was purified from BZKO cells 48 hrs after transfection with vector, wt ZEBRA or the indicated replication defective ZEBRA mutants. Expression of the brlf1 (panel A) and the gapdh (panel B) messages was measured by quantitative RT-PCR and normalized to the level of these transcripts in BZKO cells transfected with empty vector. Fold expression of brlf1 mRNA was corrected for the amount of gapdh transcripts detected in the same sample.