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Renin-Angiotensin System Components in Human Gingiva.

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posted on 05.08.2015 by Carlos F. Santos, Ana C. Morandini, Thiago J. Dionísio, Flávio A. Faria, Marta C. Lima, Caio M. Figueiredo, Bella L. Colombini-Ishikiriama, Carla R. Sipert, Rubens P. Maciel, Ana P. Akashi, Gabriela P. Souza, Gustavo P. Garlet, Camila O. Rodini, Sandra L. Amaral, Christiane Becari, Maria C. Salgado, Eduardo B. Oliveira, Isaac Matus, Daniela N. Didier, Andrew S. Greene

A) qPCR analysis of the following RAS components extracted from human gingiva from healthy (open bars), gingivitis (gray bars) and periodontitis (black bars) groups: angiotensinogen (AGT), angiotensin converting enzyme (ACE), angiotensin converting enzyme 2 (ACE-2), renin, angiotensin II receptor type 1 (AT1R), and Mas receptor (MasR), (n = 7). Graphs displays relative expression levels of the target mRNA relative to RPL-13 mRNA. The means were compared using a 1-way ANOVA and Tukey’s test. B) Immunoreactivity of AT1R and AT2R in the gingiva from healthy volunteers (open bars) and volunteers with gingivitis and periodontitis combined (black bars). The means were compared using a 1-way ANOVA and Ang with gingivitis and periodontitis is indicated by *. C) Representative photographs of immunoreactivity for either AT1R (Ca and Ce) or AT2R (Ce and Cf) in the gingiva from either healthy volunteers (Ca and Cd) or volunteers with gingivitis and periodontitis (Ce and Cf). Photographs Cb and Cd are from tissue incubated with nonimmune serum in Healthy Tissue. Scale bars indicate a distance of 20μm.