Relative quantification of AmelTwdl1, AmelTwd2 and Ampxd transcripts in different tissues of the Pbl pharate adult.

The Amrp49 gene was used as endogenous control in real-time RT-PCR assays. The relative amount of transcripts is given by 2^-ΔΔC_T. Columns and bars represent means±SE of three independent samples prepared with each tissue. AmelTwdl1 transcript levels were significantly different among the tested tissues, with a higher expression in the gut (p = 0.002). Similarly, AmelTwdl2 showed a significantly higher expression in the gut than in integument and trachea (p = 0.010). In contrast, Ampxd showed a higher expression in integument than in the other tissues (p = 0.001). Statistical analysis was carried out with Jandel SigmaStat 3.1 software (Jandel Corporation, San Rafael, CA, USA). One Way Anova; post-hoc comparisons using Holm Sidak test p<0.05. Different letters above columns indicate statistical difference.