Regulation of cardiomyocyte SgII expression by important hormonal and paracrine factors in HF.

SgII mRNA levels were measured by RT-qPCR after stimulating neonatal rat cardiomyocytes for 24 h with either PBS (Ctr, n = 9), forskolin (FSK n = 5), norepinephrine (NE, n = 5), angiotensin II (AngII, n = 4), endothelin-1 (ET-1, n = 5), transforming growth factor-β (TGF-β, n = 6), or tumor necrosis factor-α (TNF-α, n = 6). SgII mRNA levels are presented as fold change ± SEM vs. PBS-stimulated cells. **p<0.001, *p<0.01, # p<0.05.