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Regulated silencing of hStau1 protein in neuroblastoma cells.

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posted on 25.11.2014 by Joan Peredo, Patricia Villacé, Juan Ortín, Susana de Lucas

Cultures of neuroblastoma SH-SY5Y cells were transduced with a lentiviral construct able to express, under a doxicyclin inducible promoter, RFP and an shRNA with a hStau1 specific silencer (siStau) or an empty shRNA as a control (Ctrl). (A) Combined phase-contrast and fluorescence images of cells treated (bottom) or untreated (top) with doxicyclin. Bar scale correspond to 10 µm. (B) Cell extracts were obtained from the cultures described above and analysed by Western-blot using antibodies specific for hStau1 or ß-actin.

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