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Reducing Blk expression in B6.lpr mice increases numbers of IFNγ-, IL-17A-, and IL-21-producing T cells.

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posted on 2014-03-17, 03:20 authored by Elizabeth M. Samuelson, Renee M. Laird, Amber M. Papillion, Arthur H. Tatum, Michael F. Princiotta, Sandra M. Hayes

(A) Graph comparing the number of splenic Tfh cells, defined as CD4+ CXCR5+ CD40L+ cells, between 3-month-old B6 (n = 6) and Blk+/− (n = 6) mice and between 3-month-old B6.lpr (n = 7) and Blk+/−.lpr (n = 8) mice. (B) pLN cells from 3-month-old B6, Blk+/−, B6.lpr and Blk+/−.lpr were stimulated with Leukocyte Activation Cocktail for 4 hours. Representative dot plots showing CD3 versus IL-17A expression in CD4+ αβ T cells (top), γδ T cells (middle) and in DN αβ T cells (bottom) from B6, Blk+/−, B6.lpr and Blk+/−.lpr mice. Numbers represent percentages of IL-17A+ cells. (C) Graph summarizing data in (B). Comparison of the number of IL-17A+ cells per T cell subset between 3-month-old B6 (n≥4) and Blk+/− (n≥5) mice and between 3-month-old B6.lpr (n≥7) and Blk+/−.lpr (n≥8) mice. (D) Splenocytes from 3-month-old B6, Blk+/−, B6.lpr and Blk+/−.lpr mice were stimulated with Leukocyte Activation Cocktail for 4 hours. Representative dot plots show CD3 versus IFNγ expression in gated CD4+, CD8+, DN αβ and γδ T cell subsets. Numbers represent percentages of IFNγ+ cells. (E) Graph summarizing data in (D). Comparison of the percentage of IFNγ+ cells per T cell subset between 3-month-old B6 (n = 4) and Blk+/− (n = 4) mice and between 3-month-old B6.lpr (n = 5) and Blk+/−.lpr (n = 5) mice. (F) Graph comparing the numbers of RORγt+ CCR6+ γδ T cells (γδ-17 cells) and DN αβ T cells (DN-17 cells) between the pLNs of 3-month-old B6 (n = 8) and Blk+/− (n = 7) mice and between the pLNs of 3-month-old B6.lpr (n = 8) and Blk+/−.lpr (n = 12) mice. *p ≤ 0.05; **p ≤ 0.01; #p ≤ 0.001.

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