Reduced proliferation and neuronal differentiation in neurospheres isolated from adult C/EBPβ−/− compared to C/EBPβ+/+ mice.
(A) Representative Western blot showing the presence of the different C/EBPβ isoforms (LAP1, LAP2 and LIP) in NS cultures established from adult mice. (B) Immunocytochemistry analysis showing the BrdU incorporation in C/EBPβ−/− and C/EBPβ+/+ NS cultures plated for 24 h in proliferation medium. Quantification analysis show a significantly reduction in the number of BrdU-positive cells in C/EBPβ−/− cultures compared to wild type NS cultures. (C) C/EBPβ−/− and C/EBPβ+/+ NS cultures were plated for 72 h in differentiation medium and MAP2 immunocytochemistry was performed as described in Material and Methods. Quantification analysis shows that the percentage of MAP2 positive cells in C/EBPβ−/− NS cultures was significantly reduced. Nuclei were counterstained with Hoechst (blue). Scale bars, 25 µm. Values represent the mean ± S.E. **P≤0.01.