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Reduced Mena expression induces lamellipodia formation in various cell lines.

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posted on 2009-03-11, 01:37 authored by Morihiro Higashi, Chieko Ishikawa, Jianyong Yu, Akihiro Toyoda, Hidetada Kawana, Kazuo Kurokawa, Michiyuki Matsuda, Motoo Kitagawa, Kenichi Harigaya

(A–C) Morphological change in U251MG (A), U373MG (B), HeLaS3 cells (C). U251MG and U373MG spread with the introduction of hMena siRNA640. (D) Box and whisker plot for relative cell area. (E) Box and whisker plot for relative perimeter. Data is from 50 cells each. The mean perimeter of U251MG cells transfected with control siRNA was set as 1. (F) Box and whisker plots for percent lamellipodial length of the perimeter. For box and whisker plots, the top and bottom of the box represent the 75th and 25th quartile, and whiskers 10th and 90th percentiles, respectively. The middle line of the box is the median. Brackets with asterisks indicate statistically significant differences between the data sets from a Student's t test (p<0.001).

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