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Redox interactions involing human SelO.

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posted on 21.04.2014 by Seong-Jeong Han, Byung Cheon Lee, Sun Hee Yim, Vadim N. Gladyshev, Seung-Rock Lee

(A) Protein sequence alignment of the C-terminal region of SelO. (B) Recombinant SelOs. The plasmid construct was designed to trap the interacting partner. The mitochondrial leader sequence (MLS) was flanked by the HA-TEV sequence and the CXXU was changed to SXXU, CXXC, SXXC, CXXS, or SXXS. (C–D) Western blotting of SelO with (C) the cell lysates or (D) the samples that were prepared by using immunoprecipitation with anti-HA antibody from HEK293T cells expressing recombinant human SelO or its mutant forms (CXXU, CXXC, CXXS, SXXU, SXXC, SXXS).

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