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Recombined Purkinje neurons display a different miRNA profile compared to nonrecombined neighboring neurons.

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posted on 03.06.2014, 02:43 by Kirsten Ridder, Sascha Keller, Maria Dams, Anne-Kathleen Rupp, Jessica Schlaudraff, Domenico Del Turco, Julia Starmann, Jadranka Macas, Darja Karpova, Kavi Devraj, Candan Depboylu, Britta Landfried, Bernd Arnold, Karl H. Plate, Günter Höglinger, Holger Sültmann, Peter Altevogt, Stefan Momma

(A) Example for an X-Gal–positive neuron and corresponding nonrecombined Purkinje neuron that were collected by LCM for miRNA analysis. Cells were identified based on morphology and location in the Purkinje cell layer. Nonrecombined Purkinje neurons in the direct vicinity were cut out as references, depicted below. (B) Pairwise comparison of nonrecombined versus recombined Purkinje neurons from the same animal. Each symbol represents an individual miRNA; colored symbols represent miRNAs that could only be detected in one pool. Symbols representing higher expressed miRNAs are at the top; those representing lower expressed miRNAs are at the bottom of each column. The comparison of recombined versus nonrecombined PKNs from the same animals reveals significant differences in their miRNA profile. (C) Venn diagram depicting miRNAs specific for recombined PKNs. The three miRNAs displayed in the box are those that can be found in both samples of the recombined PKNs but not in any sample of the nonrecombined PKN. (D) EVs isolated from the serum of mice with peritonitis display a different miRNA content than those of healthy control animals.