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Recognition profile of STAg proteins using polyclonal antibodies raised in chicken and mice.

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posted on 12.07.2012, 00:19 by Álvaro Ferreira Júnior, Fernanda M. Santiago, Murilo V. Silva, Flávia B. Ferreira, Arlindo G. Macêdo Júnior, Caroline M. Mota, Matheus S. Faria, Hercílio H. Silva Filho, Deise A. O. Silva, Jair P. Cunha-Júnior, José R. Mineo, Tiago W. P. Mineo

(A) Differential reactivity to Toxoplasma gondii soluble antigens (STAg) of egg IgY antibodies and mouse serum IgG from animals immunized by intramuscular (i.m.) and subcutaneous (s.c.) routes initially was assessed by 1D immunoblotts (WB1D). Antigens with approximately (∼) 30 kDa (blue box) were evenly recognized by all antibodies tested, which was not observed in antigens with ∼40 kDa (green box) and ∼50 kDa (red box), which were mainly recognized by chicken and mouse antibodies, respectively. The same recognition pattern against STAg proteins was noted in two-dimensional immunoblotts (WB2D), probed with chicken IgY (B) or mouse IgG (C). (D) IgY antibodies obtained from eggs of chickens immunized with T. gondii, as well as IgY against Neospora caninum and Eimeria spp., were assayed against STAg-immobilized nitrocellulose membranes in order to check possible antibody cross-reactivity.