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Recognition of rTc_11623.20 and rTc_N_10421.310 by the sera from chronic chagasic patients or Leishmania-infected individuals.

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posted on 16.09.2014, 03:04 by João Luís Reis-Cunha, Tiago Antônio de Oliveira Mendes, Rodrigo de Almeida Lourdes, Daihana Rodrigues dos Santos Ribeiro, Ricardo Andrez Machado-de-Avila, Maykon de Oliveira Tavares, Denise Silveira Lemos, Antônia Cláudia Jácome Câmara, Carlos Chavez Olórtegui, Marta de Lana, Lúcia Maria da Cunha Galvão, Ricardo Toshio Fujiwara, Daniella Castanheira Bartholomeu

Sera from chronic chagasic patients and sera from patients with cutaneous and visceral leishmaniasis were assayed by ELISA using rTc_11623.20 (A, D), rTc_N_10421.310 (B, E) and pooled recombinant proteins (C, F). Tc-non typed, sera from chronic chagasic patients; TcII, sera from patients infected with T. cruzi TcII DTU; TcVI, sera from patients infected with T. cruzi TcVI DTU, Tc-Ind, sera from chagasic patients in the indeterminate form of Chagas disease; Tc-Card, sera from chagasic patients in the cardiac stage of Chagas disease; CL, sera from patients with cutaneous leishmaniasis; VL, sera from patients with visceral leishmaniasis; C-, uninfected individuals. Squares represent sera from chagasic patient in the initial cardiac stage. The dotted line represents the cutoff value that was obtained based on the ROC curve. The solid line corresponds to the mean values.

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