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Recognition of rTc_11623.20 and rTc_N_10421.310 by sera from C57BL/6 mice chronically infected with different T. cruzi strains.

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posted on 16.09.2014, 03:04 by João Luís Reis-Cunha, Tiago Antônio de Oliveira Mendes, Rodrigo de Almeida Lourdes, Daihana Rodrigues dos Santos Ribeiro, Ricardo Andrez Machado-de-Avila, Maykon de Oliveira Tavares, Denise Silveira Lemos, Antônia Cláudia Jácome Câmara, Carlos Chavez Olórtegui, Marta de Lana, Lúcia Maria da Cunha Galvão, Ricardo Toshio Fujiwara, Daniella Castanheira Bartholomeu

Sera from mice chronically infected with CL Brener (CL-B), Colombiana (Col) or Y (Y) T. cruzi strains, mice infected with T. rangeli (T.rang) or uninfected mice (C-) were screened by ELISA with rTc_11623.20 (A), rTc_N_10421.310 (B) or with a pool of these two recombinant proteins (C). The dotted line represents the cutoff value that was obtained based on the ROC curve. The solid line corresponds to the mean values. Col, mice infected with Colombiana; Y, mice infected with the Y strain; CL-B, mice infected with the CL Brener; T. rang, T. rangeli-infected mice. C-, uninfected mice.