Real-time PCR analysis of Brassica napus genes LEAFY COTYLEDON1 (LEC1), LEAFY COTYLEDON2 (LEC2), FUSCA3 (FUS3) involved in oil synthesis regulation and ALTERED TRYPTOPHAN REGULATION1 (ATR1) involved in glucosinolate synthesis.
The expression levels were measured in developing seeds 30 days after pollination (DAP). The relative expression level of each gene was normalized using BnACTIN2 as an internal control. Vertical bars represent the standard deviation of the mean treatments (n = 3). Asterisks indicate that mean values are significantly different between the 35S:MIR394 or 35S:m6LCR plants and WT (p < 0.05).