Range of detection of Pf Oocyst by ECL-SB.

A: Two-fold dilutions of Pf Oocysts were transferred onto a nitrocellulose membrane using a slot blot apparatus and probed with the mAb 2A10. The amount of protein was determined by measuring the intensity of a specific band using ImageJ software and was represented as integrated optical density (IOD). B: A standard curve based on the IOD values was derived using the Michaelis–Menten hyperbolic function. The linear range of antigen–antibody reaction shown in the inset is obtained within 1–4 Pf Oocysts.