Figure_5.tif (733.5 kB)
Download file

Range of detection of Pf Oocyst by ECL-SB.

Download (0 kB)
figure
posted on 2014-12-22, 14:42 authored by Sanjai Kumar, Hong Zheng, Bingbing Deng, Babita Mahajan, Bryan Grabias, Yukiko Kozakai, Merribeth J. Morin, Emily Locke, Ashley Birkett, Kazutoyo Miura, Carole Long

A: Two-fold dilutions of Pf Oocysts were transferred onto a nitrocellulose membrane using a slot blot apparatus and probed with the mAb 2A10. The amount of protein was determined by measuring the intensity of a specific band using ImageJ software and was represented as integrated optical density (IOD). B: A standard curve based on the IOD values was derived using the Michaelis–Menten hyperbolic function. The linear range of antigen–antibody reaction shown in the inset is obtained within 1–4 Pf Oocysts.

History