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Raman spectroscopy of PAVICs and MOBs in culture compared through univariate and multivariate statistical analysis.

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posted on 2012-10-26, 00:57 authored by Kristy L. Cloyd, Ismail El-Hamamsy, Suwimon Boonrungsiman, Martin Hedegaard, Eileen Gentleman, Padmini Sarathchandra, Francesca Colazzo, Molly M. Gentleman, Magdi H. Yacoub, Adrian H. Chester, Molly M. Stevens

A - Raman spectra comparing a representative MOBs mineralized nodule spectrum (black), PAVICs grown in CTL media mean spectrum (dark grey), and PAVICs cultured in OST+TGF-β1 medium mean spectrum (shaded box over entire spectrum). Vertical shaded areas highlight the 960 cm−1 apatite peak and the 1070 cm−1 carbonate peak spectral range. B - Higher magnification of the PAVICs mean spectrum grown in CTL (dark grey) and in OST+TGF-β1 medium (light grey). The red spectrum is the difference between the two PAVICs mean spectra. Shaded red bands highlight spectral ranges which discriminate between groups. C - Scatter plot showing group separation between PAVICs grown in CTL medium (red triangles) vs. PAVICs grown in OST+TGF-β1 medium (green circles). D - Latent variables loadings used in the interval partial least squares discriminant analysis (iPLS-DA) model, bands highlighted are spectral ranges which contributed to the distinction between groups.


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