Rac activation in response to p114RhoGEF depletion in MDA-MB-231 cells.
(A) MDA-MB-231 cells were transfected with the indicated siRNAs and the levels of active Rac were determined using a G-LISA assay 72 hours after transfection. Shown are means ± 1SD, n = 6. The numbers were normalized to control siRNA transfections. (B,C) Cells that had been transfected with siRNAs as in panel A were fixed and processed for immunofluorescence using an antibody specific for active Rac1 and fluorescent phalloidin. The intensity of the active Rac1 staining was quantified using image J. Panel C shows means ± 1SD of three experiments (in each experiment, at least 5 fields were analyzed).