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Rab1b knockdown inhibits the survival of Y. pestis within macrophages.

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posted on 23.10.2015, 02:45 by Michael G. Connor, Amanda R. Pulsifer, Christopher T. Price, Yousef Abu Kwaik, Matthew B. Lawrenz

RAW264.7 macrophages were reverse transfected with Rab1a, Rab1b, scrambled (Scr), or Copβ1 siRNA. 48 h after transfection cells were infected with Y. pestis (MOI 10). (A) Percent survival of intracellular CO92 pCD1(-) LuxPtolC in Rab1a or Rab1b siRNA treated macrophages as compared to Scr siRNA treated macrophages. (B) Bioluminescence of intracellular bacteria from macrophages infected for 10 h with Y. pestis CO92 pCD1(-) LuxPtolC. (C) Conventional enumeration of intracellular bacteria from macrophages infected for 10 h with Y. pestis CO92 pCD1(-) LuxPtolC. (D) Bioluminescence of intracellular bacteria from macrophages infected for 2 h with Y. pestis CO92 pCD1(-) LuxPtolC. (E) Bioluminescence of intracellular bacteria from macrophages infected for 10 h with Y. pestis KIM D-19 LuxPtolC. (F) Conventional enumeration of intracellular bacteria from macrophages infected for 10 h with Y. pestis KIM D-19 LuxPtolC. (G) Bioluminescence of intracellular bacteria from macrophages infected for 2 h with Y. pestis KIM D-19 LuxPtolC. The limit of detection for conventional enumeration is denoted by the dotted line. RLU = Relative Light Units; CFU = Colony Forming Units. *** = p<0.001, **** = p<0.0001.

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